膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8), premium grade (CD6-CH29G1) is recombinantly produced from CHO cells.
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
亚型（Isotype）
Mouse IgG1 | Mouse kappa
偶联（Conjugate）
Unconjugated
特异性（Specificity）
This product is a specific antibody that specifically reacts with CD16/Fc gamma RIII.
内毒素（Endotoxin）
Less than 0.002 EU per μg by the LAL method.
蛋白A残留（Protein A）
<5 ppm of protein tested by ELISA.
宿主蛋白残留（Host Cell Protein）
<0.5 ng/μg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
<0.02 ng/μg of protein tested by qPCR.
纯度（Purity）
>95% as determined by SDS-PAGE.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
For long term storage, the product should be stored at liquid state at -70°C.
This product is stable after storage at:
- -70°C for 24 months under sterile condition;
- 2-8℃ for 12 months under sterile condition.
质量管理控制体系（QMS）
电泳（SDS-PAGE）
Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8), premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-CELL BASE
Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone 3G8), premium grade (Cat. No. CD6-CH29G1) stimulates Human CD16a (158V) (Luc) Jurkat Reporter Cell. The typically EC50 for this effect is 57.70 ng/mL (QC tested).
Protocol
活性（Bioactivity）-ELISA
Immobilized Human CD16a (V176), His Tag (Cat. No. CD8-H52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8), premium grade (Cat. No. CD6-CH29G1) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-FACS
Flow cytometric analysis of Human peripheral blood mononuclear cell respectively staining with Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8), premium grade (Cat. No. CD6-CH29G1) at 10 μg/mL (1 μg of the antibody corresponds to labeling of 1e6 cells in a final volume of 100 µL) and PE anti-mouse IgG1 Antibody, compared with isotype control antibody. PE signal was used to evaluate the binding activity (Routinely tested).
Protocol
产品推荐（Recommended Products）
背景（Background）
CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
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