膜杰作
Star Staining
描述（Description）
HEK293/Human Tau-K18 (GFP) Stable Cell Line
应用说明（Application）
Tau PFF induced intracellular aggregation assay
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Hygromycin B (250 μg/mL)
培养基（Culture Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
10% DMSO + 90% FBS
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
FACS analysis of Tau-K18 (GFP) on HEK293/Human Tau-K18 (GFP) Stable Cell Line.
HEK293/Human Tau-K18 (GFP) Stable Cell Line was red line, negative control HEK293 cells was grey line (QC tested).
Protocol
活性（Bioactivity）-Bioactivity CELL BASE
HEK293/Human Tau-K18 (GFP) Stable Cell Line (Cat. No. CHEK-ATP087) were transduced with Human Tau-441 Pre-formed Fibrils, Tag Free (Cat. No. TAU-H5115) and Human Tau-441, Tag Free (Cat. No. TAU-H5117) respectively. The fluorescence of GFP-Tau (Green) and DAPI (Blue) were detected by confocal microscope. A. Lipo2000 transduction. B. Lipo2000 and Human Tau-441, Tag Free transduction. C. Lipo2000 and Human Tau-441 Pre-formed Fibrils, Tag Free transduction. Scale bars, 50 μm (Routinely tested).
Protocol
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背景（Background）
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
